Till Bachmann and Kate Templeton win poster prize at NHS Research Scotland Conference

Many congratulations to Edinburgh Infectious Diseases members Till Bachmann and Kate Templeton

Their poster entitled "Rapid electrochemical detection of multi-drug resistance NDM-1 producing bacteria" 
won the prize for Most Innovative Science at the recent NHS Research Scotland Conference.

The work was carried out in the Division of Pathway Medicine, Royal Infirmary of Edinburgh, Little France by Jimmy Ming-Yuan Huang, Grace Henihan, Daniel Macdonald, Annette Michalowski, Holger Schulze, Kate Templeton, Alan P. Gibb and Till Bachmann.  Their studies have resulted in the development of an electrochemical sensor which allows point of care detection of strains of potentially multidrug resistant gram negative bacteria. 

More details are given in the poster abstract below.

Each year, in the order of 25 000 patients die in the EU from an infection with key multidrug-resistant bacteria (Anon 2009). While until recently resistance in gram positive bacteria have been the primary focus, increasing prevalence of resistant gram negative strains, in combination with the lack of antimicrobials in development to target the issue, means that the threat posed by gram negative bacteria is now greater. The blaNDM-1 gene was first isolated from a Swedish patient previously hospitalised in India in 2008, and has disseminated to broad geographical locations. Spread of the gene is of grave concern as organisms harbouring NDM-1 tend to be multi resistant, and some are only sensitive in vitro to agents of uncertain efficacy such as tigecycline and colistin. As a result prompt identification of NDM-1 strains is of paramount importance to prevent transmission and dissemination of resistant strains.

Here, we report the successful development of an electrochemical sensor, whereby PCR targets derived from clinical isolates carrying the blaNDM-1 beta-lactam resistance gene are detected label-free using electrochemical impedance spectroscopy. The assay format is applicable to rapid point of care scenarios. NDM-1 specific probe sequences were developed in silico and validated with fluorescent glass microarray prior to electrochemical application. Using screen-printed electrodes functionalized with a NDM-1 specific probe a specific and sensitive electrochemical impedance spectroscopy assay was developed based on hybridization of synthetic oligonucleotides. The assay was applied to detection of single stranded NDM-1 PCR product in a specific manner to an LOD of 100 pM. The assay has also been demonstrated to hold potential of amplification-free detection of bacterial DNA, as reported herein.

The study was part funded by a grant from the Scottish Infection Research Network (SIRN).


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